Clostridium histolyticum contains two distinct but related genes for collagenase. The col G gene codes for a 936-amino acid protein designated Collagenase Type 1 and the col H gene codes for a 1021 amino acid protein designated Collagenase Type II. As a result of the correlations, several types of partially purified collagenases have been established by Worthington: Types 1, 2, 3, 4, 5, 6 and 7.
Worthington prepares Deoxyribonuclease I from bovine pancreas at different levels of purity to suit many different applications.
Worthington Papain is purified from Carica papaya latex. This enzyme has wide specificity, and it will degrade most protein substrates more extensively than the pancreatic proteases.
Worthington purifies trypsin from bovine pancreas. Our enzyme is extensively used in protein sequencing work and for tissue dissociation.
Worthington Elastase is prepared from porcine pancreas. It is extensively used in tissue and cell dissociation procedures.
Worthington Ribonucleases A & B are prepared from bovine pancreas and are offered in several grades to suit various applications. When Ribonuclease A is used to remove RNA during DNA isolation, the enzyme must be free of deoxyribonuclease activity to prevent damage to the DNA.
IPTG is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactosemetabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator.
X-gal is often used in molecular biology to test for the presence of an enzyme, β-galactosidase. It is also used to detect activity of this enzyme in histochemistry and bacteriology.